Uji Aktivitas Penghambatan Enzim Tirosinase Oleh Ekstrak Etanol Daun Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Secara In Vitro
Keywords:
IC50, Typhonium flagelliforme (Lodd.) Blume, tyrosinase inhibitors
Abstract
Hyperpigmentation is an event that occurs due to excessive production of skin pigment. Skin color is strongly influenced by the presence of melanin, where the presence of melanin is strongly influenced by the enzyme tyrosinase. Taro leaf (Typhonium flagelliforme (Lodd.) Blume) are one of the plants have flavonoid compounds used as antioxidants and tyrosinase inhibitors. The aim of this study was to test the ethanol extract of taro leaf as a tyrosinase inhibitor. Taro leaf in this study were obtained through an extraction process using 96% ethanol solvent by maceration method. The thick ethanol extract of taro leaf was tested for phytochemical screening, total flavonoid levels, and tyrosinase inhibition test. The method used for tyrosinase inhibition test was enzymatic method in vitro. The results of this study indicated that there was a total flavonoid compound content of 0.7378% (b/b) and the inhibitor activity can be seen from the IC50 value for the diphenolation reaction (L-DOPA substrate) which was 13.307 mg/mL. This value was greater when compared with 0.093 mg/mL of phenolic acid, so that the ethanol extract of taro leaf has the potential to inhibit tyrosinase activity.References
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Utami, N., Devy, L., & Arianto, A. (2016). Growth and Yield Response of Rodent Tuber (Typhonium flagelliforme (Lodd.) Blume) under Different Light Intensities and Concentrations of Paclobutrazol. Jurnal Jamu Indonesia, 1(3), 29–35. https://doi.org/10.29244/jjidn.v1i3.30641
Utami, Rahmi. (2014). Inhibitor Tirosinase Ekstrak Metanol Berbagai Bagian Pohon Jabon Dan Mangium. Departemen Hasil Hutan Fakultas Kehutanan Institut Pertanian Bogor Bogor.
Wimpy, & Harningsih, T. (2017). Uji Aktivitas Antioksidan Kombinasi Ekstrak Sarangsemut ( Myrmecodia Pendans ) Dan Ekstrak Keladi Tikus (Typhonium flagelliforme Lodd) Dengan Metode DPPH. Jurnal Kesehatan Kusuma Husada, 35–41.
Zakiyana, Y., Supriatno, & Medawati, A. (2010). Efek ekstrak etanol daun keladi tikus (Typhonium flagelliforme Lodd.) pada invasi sel kanker lidah manusia ( SP-C1 ) in vitro. Mutiara Medika, 10(2), 160–166.
Aprilliani, A., Suganda, A. G., & Hartati, R. (2018). Uji inhibisi Aktivitas Enzim Tirosinase Beberapa Tenis Tumbuhan Zingiberaceae. Jurnal Ilmiah Farmasi, 14(1), 46–58.
Arifianti, A. E., Anwar, E., & Nurjanah. (2017). Aktivitas Penghambatan Tirosinase Dan Antioksidan Serbuk Rumput Laut Dari Sargassum plagyphyllum Segar Dan Kering. JPHPI, 20(20), 3.
Chairunnisa, S., Wartini, N. M., & Suhendra, L. (2019). Pengaruh Suhu dan Waktu Maserasi terhadap Karakteristik Ekstrak Daun Bidara ( Ziziphus mauritiana L . ) sebagai Sumber Saponin. Jurnal Rekayasa Dan Manajemen Agroindustri, 7(4), 551–560.
Chang, T. S. (2009). An updated review of tyrosinase inhibitors. International Journal of Molecular Sciences, 10(6), 2440–2475. https://doi.org/10.3390/ijms10062440
Chatatikun, M., & Chiabchalard, A. (2017). Thai plants with high antioxidant levels, free radical scavenging activity, anti-tyrosinase and anti-collagenase activity. BMC Complementary and Alternative Medicine, 17(487), 1–9. https://doi.org/10.1186/s12906-017-1994-7
Cherian, E., Patani, G., Sudheesh, N. P., & Janardhanan, K. K. (2009). Free-radical scavenging and mitochondrial antioxidant activities of relshl-ganoderma lucjdum (curt: Fr) p. karst and arogyapacha-trichopus zeylanicus gaertn extracts. Journal of Basic and Clinical Physiology and Pharmacology, 20(4), 289–308. https://doi.org/10.1515/JBCPP.2009.20.4.289
Direktorat Jendral POM, Depkes RI. (1986). Sediaan Galenik. Departemen Kesehatan RI. Jakarta. Halaman 5,6,8-28.
Direktorat Jendral POM, Depkes RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Direktorat Jendral Pengawasan Obat dan Makanan. Jakarta. Halaman 31-32.
Goenka, S., & Toussaint, J. (2020). Citrate-Coated Platinum Nanoparticles Exhibit a Primary Particle-Size Dependent Effect on Stimulating Melanogenesis in Human Melanocytes. Cosmetics, 7(4), 88. https://doi.org/10.3390/cosmetics7040088
Hindun, S., Rusdiana, T., Abdasah, M., & Hindritiani, R. (2017). Potensi Limbah Kulit Jeruk Nipis (Citrus auronfolia) Sebagai Inhibitor Tirosinase. Indonesian Journal of Pharmaceutical Science and Technology, 4(2), 64–69.
Jakimiuk, K., Sari, S., Milewski, R., Supuran, C. T., Şöhretoğlu, D., & Tomczyk, M. (2022). Flavonoids as tyrosinase inhibitors in in silico and in vitro models: basic framework of SAR using a statistical modelling approach. Journal of Enzyme Inhibition and Medicinal Chemistry, 37(1), 421–430. https://doi.org/10.1080/14756366.2021.2014832
Jayantie, D. D., Farida, Y., & Taurhesia, S. (2022). Aktivitas Antioksidan Dan Inhibisi Enzim Tirosinase Ekstrak Etanol Buah Gandaria (Bouea macrophylla Griff.) Secara In Vitro. Pharmacoscript, 5(1), 62–70.
Katrin, E., Susanto, Winarno, H., & Novagusda, F. N. (2012). Karakteristika dan Khasiat Daun Keladi Tikus (Typhonium divaricatum (L .) Decne) Iradiasi. Jurnal Ilmiah Aplikasi Isotop Dan Radiasi, 8(1), 31–42. Retrieved from http://jurnal.batan.go.id/index.php/jair/article/view/492
Kurniasari, A., Anwar, E., & Djajadisastra, J. (2018). Potensi Ekstrak Biji Coklat (Theobroma cacao Linn) sebagai Inhibitor Tirosinase untuk Produk Pencerah Kulit. Jurnal Kefarmasian Indonesia, 8(1), 34–43. https://doi.org/10.22435/jki.v8i1.7722.34-43
Leba, M.A.U. (2017). Buku ajar : Ekstraksi dan Real Kromatografi. Yogyakarta. Penerbit Deepublish, Desember 2017. X, 112 hlm.; Uk: 14x20 cm.
Lukitaningsih, E., Mustikawaty, A. A., & Sudarmanto, A. (2013). Homology Modeling dan Molecular Docking Senyawa Aktif dari Bengkoang ( Pachyrrhizus erosus ) sebagai Inhibitor Tirosinase pada Homo sapiens. Jurnal Ilmu Kefarmasian Indonesia, 11(2), 134–141.
Mawaddah, M., & Susilawati, Y. (2018). Review Artikel: Potensi Tumbuhan Sebagai Whitening Agent. Farmaka, 16(2), 598–605.
Nur, S., Rumiyati, R., & Lukitaningsih, E. (2017). Skrining Aktivitas Antioksidan, Antiaging Dan Penghambatan Tyriosinase Dari Ekstrak Etanolik Dan Etil Asetat Daging Buah Dan Kulit Buah Langsat (Lansium domesticum Corr) Secara In Vitro. Traditional Medicine Journal, 22(1), 63–72.
Sari, D. Y., R, W., & AN, T. (2021). Penentuan Kadar Flavonoid Total Ekstrak Etanol Jamur Susu Harimau (Lignosus rhinocerus). Jurnal Farmasi Udayana, 10(1), 23–30. https://doi.org/10.24843/jfu.2021.v10.i01.p03
Sianipar, N. F., Purnamaningsih, R., & Rosaria. (2016). Pengembangan Tanaman Keladi Tikus (Typhonium Flagelliforme L.) Asal Indonesia Sebagai Obat Antikanker. Jurnal Penelitian Dan Pengabdian Masyarakat, 4(1), 65–74.
Siregar, I. D., Kusuma, H. S. W., Widowati, W., Marpaung, H. H., Ferdinand, S., Fachrial, E., & Lister, I. N. E. (2019). Antioxidant and Antityrosinase Activities of Ethanolic Pachyrhizuserosus Peel and Tuber Extract. Majalah Kedokteran Bandung, 51(2), 75–81. https://doi.org/10.15395/mkb.v51n2.1628
Supriningrum, R., Nurhasnawati, H., & Medina, P. (2017). Penetapa Kadar Flavonoid Ekstrak Etanol Umbi Bawang Tiwai (Eleutherine palmifolia L. Merr) Berdasarkan Ukuran Serbuk Simplisia. Media Sains, 10(1), 42–46.
Syafruddin. (2018). Pengaruh Ekstrak Daun Keladi Tikus (Typhonium flagelliforme) Terhadap Aktivitas Antimutagenik Pada Mencit (Mus musculus) Dengan Menggunakan Metode Mikronukleus Assay. Media Farmasi, 16(1), 35–44.
Utami, N., Devy, L., & Arianto, A. (2016). Growth and Yield Response of Rodent Tuber (Typhonium flagelliforme (Lodd.) Blume) under Different Light Intensities and Concentrations of Paclobutrazol. Jurnal Jamu Indonesia, 1(3), 29–35. https://doi.org/10.29244/jjidn.v1i3.30641
Utami, Rahmi. (2014). Inhibitor Tirosinase Ekstrak Metanol Berbagai Bagian Pohon Jabon Dan Mangium. Departemen Hasil Hutan Fakultas Kehutanan Institut Pertanian Bogor Bogor.
Wimpy, & Harningsih, T. (2017). Uji Aktivitas Antioksidan Kombinasi Ekstrak Sarangsemut ( Myrmecodia Pendans ) Dan Ekstrak Keladi Tikus (Typhonium flagelliforme Lodd) Dengan Metode DPPH. Jurnal Kesehatan Kusuma Husada, 35–41.
Zakiyana, Y., Supriatno, & Medawati, A. (2010). Efek ekstrak etanol daun keladi tikus (Typhonium flagelliforme Lodd.) pada invasi sel kanker lidah manusia ( SP-C1 ) in vitro. Mutiara Medika, 10(2), 160–166.
Published
2023-01-26
How to Cite
Sholikha, M., Wahyuningtyas, W., & Puspitasari, L. (2023). Uji Aktivitas Penghambatan Enzim Tirosinase Oleh Ekstrak Etanol Daun Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Secara In Vitro. Sainstech Farma: Jurnal Ilmu Kefarmasian, 16(1), 1-6. https://doi.org/https://doi.org/10.37277/sfj.v16i1.1451
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Articles
