Sainstech Farma: Jurnal Ilmu Kefarmasian

Effectiveness Test of Anti-Inflammatory Properties of Jamblang Leaf Extract (Syzygium cumini (L.) Against Carrageenan-induced Inflammation in Mice (Mus musculus)

Oktaviana Zunnita, Moerfiah Moerfiah, Risa Auliya — Tue, 16 Jan 2024 11:46:15 +0000

Inflammation is a protective process against tissue injury caused by infection, trauma, or chemicals. The mechanism of inflammation starts from a stimulus that will cause cell damage, these cells will release several phospholipids, one of which is arachidonic acid. Free arachidonic acid will be activated by cyclooxygenase and lipoxygenase enzymes. This enzyme converts arachidonic acid into an unstable form which is further metabolized to leukotrienes, prostaglandins, prostacyclin, and thromboxane. One of the natural medicines used as an inflammatory treatment is jamblang leaf, because there are flavonoids that can inhibit cyclooxygenase and lipoxygenase enzymes. This study aims to determine the anti-inflammatory effect and the best dose of jamblang leaf extract. The method used was 1% carrageenan induction and a digital plethysmometer to measure the volume of inflammation on the soles of the test animals' feet. The test animals used were 25 mice divided into 5 treatment groups, each group consisting of 5 mice. The treatment group consisted of: negative control of 1% Na-CMC, positive control of Diclofenac Sodium 0.182 mg/20 g BW, dose 1 of jamblang leaf extract 10 mg/20 g BW, dose 2: 15 mg/20 g BW and dose 3: 20 mg/kg 20 g BB. The results showed that all doses of jamblang leaf extract had an anti-inflammatory effect and dose 3 was the best dose that reduced the volume of inflammation.

Antibacterial Activity of 96% Ethanol Extract of Seeds Petai Cina Plant (Leucaena leucocephala (Lam.) de Wit) Against Bacillus subtilis ATCC 6633

Tiara Teonanda, Muchammad Reza Ghozaly, Inherni Marti Abna — Tue, 16 Jan 2024 15:19:23 +0000

Petai cina is a plant that is often used as traditional medicine among the community. This plant has been reported to have pharmacological activity as antibacterial, antidiabetic, anti-inflammatory, anticancer, anthelmintic, antioxidant and larvicide. The seeds of the Chinese petai are often used as a traditional treatment for wounds and swelling. This research aims to determine the antibacterial activity of 96% ethanol extract of Chinese petai seeds against the bacteria Bacillus subtilis ATCC 6633. In this study, extraction was carried out using the ultrasonic assisted method (UAE), testing the antibacterial activity using the agar diffusion method using the well method. The results of phytochemical screening showed that the 96% ethanol extract of Chinese petai seeds contained flavonoids, saponins, tannins and triterpenoids. The 96% ethanol extract of petai china seeds has antibacterial activity against Bacillus subtilis ATCC 6633 which was indicated by the formation of a clear zone around the well. The 96% ethanol extract of petai chinese seeds has moderate antibacterial power against Bacillus subtilis ATCC 6633 with an inhibition zone diameter of between 5 mm-10 mm with 50 μl of extract was taken from various concentrations (300, 350, 400, 450, 500 mg/mL).

Analysis of Rhodamine B Levels in Blush On Circulating Via Online Shops Using the TLC Method and UV-Vis Spectrophotometry

Devi Ananda Putri, Fadilah Qonitah, Ahwan Ahwan — Fri, 23 Feb 2024 00:07:37 +0000

Rhodamine B is a synthetic dye used as a paper and textile dye. Rhodamine B is often misused as a dye in cosmetic preparations, one of which is blush on. Long-term use of Rhodamine B in cosmetic preparations will result in cancer and impaired liver function. If exposed to large amounts of Rhodamine B, acute symptoms of poisoning will occur within a short time. This study aims to analyze whether blush on sold through online shops in the Surakarta area contains Rhodamin B. Analysis of Rhodamine B levels used 11 blush on samples purchased through online shops including Shopee, Lazada and Tokopedia. The testing method is carried out with two tests, namely qualitative and quantitative tests. The qualitative test uses Thin Layer Chromatography (TLC) and the quantitative test uses the UV-Vis Spectrophotometry method. Based on the results of research analysis of Rhodamine B levels in blush on sold through online shops in the Surakarta area, of the 11 samples tested, there were 2 blush on samples that were declared positive for containing Rhodamine B, namely sample A and sample C. The respective levels of Rhodamine B in sample A amounted to 797.8 ± 0.92 µg/mL, while sample C was 1,047.20 ± 1.16 µg/mL. In this study it can be concluded that there are 2 blush samples that do not meet the requirements of the Regulation of the Minister of Health of the Republic of Indonesia No.722/Menkes/Per/IX/1998 and the Director General of Drug and Food Control No.00386/C/SK/II/1990.

Evaluasi Rasionalitas Penggunaan Obat Diare Pada Balita Di Rumah Sakit Umum Daerah Kota Depok Periode Januari-Desember 2021

Ritha Widyapratiwi, Yayah Siti Djuhariah, Arnetha Octavia Laurent — Sat, 24 Feb 2024 04:40:53 +0000

Diare akut adalah diare yang terjadi dalam waktu kurang dari 14 hari. Diare mengakibatkan kematian tertinggi pada kelompok balita usia 12-59 bulan. Penyakit diare juga masih menjadi 10 penyakit terbanyak yang ditangani oleh Rumah Sakit di Jawa Barat. Penelitian ini bertujuan untuk melakukan evaluasi terhadap rasionalitas penggunaan obat diare akut pada pasien balita di Rumah Sakit Umum Daerah Kota Depok pada periode Januari-Desember 2021 berdasarkan kriteria tepat obat, tepat indikasi, tepat dosis, tepat cara pemberian, dan tepat lama pemberian. Metode penelitian ini adalah observasional deskriptif dengan pendekatan secara restrospektif. Teknik pengumpulan data dilakukan dengan menggunakan total sampling sebanyak 61 rekam medis yang terdiri dari diare non-spesifik dan diare spesifik. Hasil penelitian menunjukkan diare non spesifik tepat indikasi sebesar 100,00%, tepat obat 96,73%, tepat dosis 98,37 %, tepat cara pemberian 100,00%, dan tepat lama pemberian 98,37%. Diare spesifik menunjukkan persentase tepat indikasi sebesar 100%, tepat obat 86,89 %, tepat dosis 98,37%, tepat cara pemberian 100,00%, dan tepat lama pemberian 91,81%.

Rationality Evaluation of Antibiotics Use in Patients with Acute Respiratory Tract Infections at the Pengandonan Health Center Pagaralam City

Ainun Wulandari, Anggi Irma Yani Oktari, Vilya Syafriana — Fri, 17 May 2024 18:17:14 +0000

Acute Respiratory Tract Infections (ARTI) are one of the most common causes of consultation or treatment in health care facilities, especially in pediatric services. If not treated properly, upper respiratory tract infections can develop into lower respiratory tract infections. Therefore, rational treatment is necessary. This study aims to determine patient characteristics, diagnosis, drug use, and rationality for the use of antibiotics in ARTI patients at the Pengandonan Health Center, Pagaralam City in 2022 based on the right drug, right dose, right interval of administration, and right duration of administration criteria. This research is a descriptive observational study, with retrospective data collection. The sample used in this study was 95 patients, sampling was carried out using systematic random sampling technique. The results of this study showed that the highest number of ARTI characteristics are women (51.57%) and the largest age group is toddlers (0-5 years) at 29.47%. The most common diagnosis was common cold (51.57%) and the most common antibiotic was amoxicillin (73.68%). The results of the rationality of antibiotic use based on the right drug were 81.05%, 87,83% of the right dose, 97.40% of the right interval of administration, and 16.21% of the right time of administration.