Effectiveness Test of Anti-Inflammatory Properties of Jamblang Leaf Extract (Syzygium cumini (L.) Against Carrageenan-induced Inflammation in Mice (Mus musculus)
Keywords:
anti-inflammatory, jamblang leaf extract, mice
Abstract
Inflammation is a protective process against tissue injury caused by infection, trauma, or chemicals. The mechanism of inflammation starts from a stimulus that will cause cell damage, these cells will release several phospholipids, one of which is arachidonic acid. Free arachidonic acid will be activated by cyclooxygenase and lipoxygenase enzymes. This enzyme converts arachidonic acid into an unstable form which is further metabolized to leukotrienes, prostaglandins, prostacyclin, and thromboxane. One of the natural medicines used as an inflammatory treatment is jamblang leaf, because there are flavonoids that can inhibit cyclooxygenase and lipoxygenase enzymes. This study aims to determine the anti-inflammatory effect and the best dose of jamblang leaf extract. The method used was 1% carrageenan induction and a digital plethysmometer to measure the volume of inflammation on the soles of the test animals' feet. The test animals used were 25 mice divided into 5 treatment groups, each group consisting of 5 mice. The treatment group consisted of: negative control of 1% Na-CMC, positive control of Diclofenac Sodium 0.182 mg/20 g BW, dose 1 of jamblang leaf extract 10 mg/20 g BW, dose 2: 15 mg/20 g BW and dose 3: 20 mg/kg 20 g BB. The results showed that all doses of jamblang leaf extract had an anti-inflammatory effect and dose 3 was the best dose that reduced the volume of inflammation.References
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Emelda. (2019). Farmakognosi Untuk Mahasiswa Kompetensi Keahlian Farmasi. Pustaka Baru Press: Yogyakarta.
Dewi,S.T.R. & Wahyuni, S. (2018). Uji Efek Anti Inflamasi Rebusan Daun Jamblang (Syzygium cumini) Pada Mencit (Mus musculus). Media Farmasi, 14(1), 53-59.
Frida, F. (2018). Perbandingan Kadar Flavonoid Total Esktrak Etanol Daun Jamblang (Syzgium cumini (L.) Skeels ) Pada Dua Tempat Tumbuh. Skripsi, Universitas Wahid Hasyim, Semarang.
Gafur, M. (2011). Isolasi dan Identifikasi Senyawa Flavonoid dari Daun Jamblang (Syzygium cumini). Skripsi, Universitas Gorontalo, Gorontalo.
Hanani, E. (2015). Analisis Fitokimia. Jakarta: Buku Kedokteran EGC.
Katzung, B.G. (2018). Basic & Clinical Pharmacology, 10th Ed. New York: Mc Graw Hill Lange.
Kemenkes RI. (2017). Formularium Ramuan Obat Tradisional Indonesia. Jakarta: Kementerian Kesehatan Republik Indonesia.
Fitriyani, A., Winarti, L., Muslichah, S., & Nuri. (2011). Uji Antiinflamasi Ekstrak Metanol Daun Sirih Merah (Piper crocatum Ruiz & Pav) Pada Tikus Putih. Majalah Obat Tradisional, 16(1), 34-42.
Landefeld, K., Gonzales, H., & Sander, G. (2016). Hypertensive Crisis: The Causative Effects of Nonsterooidal Anti-Inflammatory Drugs. Journal of Clinical Case Reports, 6(7), 1-3.
Marjoni, R. (2016). Dasar – dasar Fitokimia. Jakarta: Trans Info Media.
Necas, J. & Bartosikova, L. (2013). Carrageenan: A Review. Veterinarni Medicina, 58, 187-205.
Nasution. (1992). Metode Research. Bandung: Jammers
Oktiwilianti,W., Umi Y,. & Ratu, C. (2015). Uji Aktivitas Antiinflamasi dari Ekstrak Etanol Daun Asam Jawa terhadap Tikus Wistar Jantan. Prosiding Farmasi SPeSIA, 1(2), 111-117.
Prasetyo & Inoriah, E. (2013). Pengelolaan Budidaya Tanaman Obat-obatan (Bahan Simplisia). Bengkulu: Badan Penerbitan Fakultas Pertanian UNIB.
Qinghu, W., Jinmei, J., Nayintai, D., Narenchaoketu, H., Jingjing, H., & Baiyinmuqier, B. (2016). Anti-Inflammatory Effects, Nuclear Magnetic Resonance Identification And HighPerformance Liquid Chromatography Isolation Of The Total flavonoids From Artemisia frigida. Journal Of Food And Drug Analysis, 24, 385-391.
Sharma, S., Mehat, B.K., Mehta, D., Nahgar, A., & Mishra, A. . (2012). A Review on Pharmacological Activity of Syzygium cumini Extracts Using Different Solvent and Their Effective Doses. International Research Journal of Pharmacy, 3(12), 54-58.
Soenarto. (2014). Inflamasi Ilmu Penyakit Dalam. Jakarta: Interna Publishing.
Sormin, R., Soukotta, D., Risambessy, A., & Ferdinan-dus, S.J. (2018). Sifat fisiko-kimia semi refined carrageenan dari kota Ambon dan Kabupaten Maluku Tenggara Barat. Pengolahan Hasil Perikanan Indonesia, 21, 92-98.
Wijaya, I.P.A., Yantini, K.E., & Susila, I.M.D.P. (2015). Faktor-faktor yang Memengaruhi Intensitas Nyeri Pasien Pasca Operasi Fraktur Ekstremitas Bawah Di BRSU Tabanan. Journal Center of Research Publication in Midwifery and Nursing, 2(1), 1-12.
Published
2024-01-16
How to Cite
Zunnita, O., Moerfiah, M., & Auliya, R. (2024). Effectiveness Test of Anti-Inflammatory Properties of Jamblang Leaf Extract (Syzygium cumini (L.) Against Carrageenan-induced Inflammation in Mice (Mus musculus). Sainstech Farma: Jurnal Ilmu Kefarmasian, 17(1), 1-8. https://doi.org/https://doi.org/10.37277/sfj.v17i1.1574
Section
Articles
